xtt cell viability kit Search Results


xtt reagent  (Biotium)
95
Biotium xtt reagent
Xtt Reagent, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xtt+cell+viability+kit/XTT+Cell+Viability+Assay+Kit/10__3390_slash_jox16020065-50-0-7
Average 95 stars, based on 1 article reviews
xtt reagent - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier
xtt cell viability kit  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc xtt cell viability kit
TSP1 and indoxyl sulfate limit <t>VSMC</t> <t>proliferation</t> via CD47. hVSMC cell viability was measured by assessing reduction in tetrazolium salt sodium 3′- [1- [(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate <t>(XTT)</t> and measuring absorbance at 450 nm in cells after 48 h treatment with ( A ) TSP1 (0, 0.2, 2.2, 5, 10 nM) ( n = 4), ( B ) TSP1 2.2 nM ± pre-treatment with anti-CD47 antibody for 30 min ( n = 6), ( C ) IS (0, 1, 10, 100, 500 µM) ( n = 4), or ( D ) IS (100, 500 µM) ± pre-treatment with anti-CD47 antibody for 30 min ( n = 4–8). All data shown are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 by one-way analysis of variance with Holm–Sidak post-hoc test ( A , C ) or Kruskal–Wallis test ( B , D ). Abbreviations: αCD47—anti-CD47 antibody; hVSMC—human aortic vascular smooth muscle cell; IS—indoxyl sulfate; TSP1—thrombospondin-1.
Xtt Cell Viability Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xtt+cell+viability+kit/XTT+Cell+Viability+Kit/pmc12840690-147-7-12
Average 95 stars, based on 1 article reviews
xtt cell viability kit - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier
colorimetric xtt cell viability and proliferation kit  (Biomol GmbH)
90
Biomol GmbH colorimetric xtt cell viability and proliferation kit
TSP1 and indoxyl sulfate limit <t>VSMC</t> <t>proliferation</t> via CD47. hVSMC cell viability was measured by assessing reduction in tetrazolium salt sodium 3′- [1- [(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate <t>(XTT)</t> and measuring absorbance at 450 nm in cells after 48 h treatment with ( A ) TSP1 (0, 0.2, 2.2, 5, 10 nM) ( n = 4), ( B ) TSP1 2.2 nM ± pre-treatment with anti-CD47 antibody for 30 min ( n = 6), ( C ) IS (0, 1, 10, 100, 500 µM) ( n = 4), or ( D ) IS (100, 500 µM) ± pre-treatment with anti-CD47 antibody for 30 min ( n = 4–8). All data shown are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 by one-way analysis of variance with Holm–Sidak post-hoc test ( A , C ) or Kruskal–Wallis test ( B , D ). Abbreviations: αCD47—anti-CD47 antibody; hVSMC—human aortic vascular smooth muscle cell; IS—indoxyl sulfate; TSP1—thrombospondin-1.
Colorimetric Xtt Cell Viability And Proliferation Kit, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xtt+cell+viability+kit/colorimetric+xtt+cell+viability+and+proliferation+kit/pm34329787-122-13-15
Average 90 stars, based on 1 article reviews
colorimetric xtt cell viability and proliferation kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
xtt cell viability assay kit  (EuroClone)
90
EuroClone xtt cell viability assay kit
DX3 and WM-115 cells were seeded as described in Materials and Methods and treated with 0.1% DMSO, melatonin (MLT), UCM 976, UCM 1032, UCM 1033 and UCM 1037 dissolved in 0.1% DMSO at the indicated doses expressed in molarity (M). DX3 <t>cell</t> <t>viability</t> was evaluated by <t>XTT</t> assay after 24 (panel A ), 48 (panel B ) and 72 hours (panel C ). Panels D – F show WM-115 cell viability evaluated as described above after 24, 48 and 72 hours respectively. Graphic bars represent percentage of living cells in each sample. The results have been normalized to 0.1% DMSO treated cells and are the means of three independent experiments ± s.d. * P -value < 0.001 versus 0.1% DMSO treated cells.
Xtt Cell Viability Assay Kit, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xtt+cell+viability+kit/xtt+cell+viability+assay+kit/pmc05620261-188-9-16
Average 90 stars, based on 1 article reviews
xtt cell viability assay kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


TSP1 and indoxyl sulfate limit VSMC proliferation via CD47. hVSMC cell viability was measured by assessing reduction in tetrazolium salt sodium 3′- [1- [(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate (XTT) and measuring absorbance at 450 nm in cells after 48 h treatment with ( A ) TSP1 (0, 0.2, 2.2, 5, 10 nM) ( n = 4), ( B ) TSP1 2.2 nM ± pre-treatment with anti-CD47 antibody for 30 min ( n = 6), ( C ) IS (0, 1, 10, 100, 500 µM) ( n = 4), or ( D ) IS (100, 500 µM) ± pre-treatment with anti-CD47 antibody for 30 min ( n = 4–8). All data shown are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 by one-way analysis of variance with Holm–Sidak post-hoc test ( A , C ) or Kruskal–Wallis test ( B , D ). Abbreviations: αCD47—anti-CD47 antibody; hVSMC—human aortic vascular smooth muscle cell; IS—indoxyl sulfate; TSP1—thrombospondin-1.

Journal: International Journal of Molecular Sciences

Article Title: Thrombospondin 1–CD47 Signalling Modulates Vascular Smooth Muscle Cell Senescence in Chronic Kidney Disease

doi: 10.3390/ijms27020755

Figure Lengend Snippet: TSP1 and indoxyl sulfate limit VSMC proliferation via CD47. hVSMC cell viability was measured by assessing reduction in tetrazolium salt sodium 3′- [1- [(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate (XTT) and measuring absorbance at 450 nm in cells after 48 h treatment with ( A ) TSP1 (0, 0.2, 2.2, 5, 10 nM) ( n = 4), ( B ) TSP1 2.2 nM ± pre-treatment with anti-CD47 antibody for 30 min ( n = 6), ( C ) IS (0, 1, 10, 100, 500 µM) ( n = 4), or ( D ) IS (100, 500 µM) ± pre-treatment with anti-CD47 antibody for 30 min ( n = 4–8). All data shown are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 by one-way analysis of variance with Holm–Sidak post-hoc test ( A , C ) or Kruskal–Wallis test ( B , D ). Abbreviations: αCD47—anti-CD47 antibody; hVSMC—human aortic vascular smooth muscle cell; IS—indoxyl sulfate; TSP1—thrombospondin-1.

Article Snippet: Cell proliferation and senescence were measured using XTT Cell Viability Kit (#9095, Cell Signalling Technology, Danvers, MA, USA) and Mammalian β-Galactosidase Assay Kit (75707, Thermo Fisher Scientific), respectively.

Techniques:

DX3 and WM-115 cells were seeded as described in Materials and Methods and treated with 0.1% DMSO, melatonin (MLT), UCM 976, UCM 1032, UCM 1033 and UCM 1037 dissolved in 0.1% DMSO at the indicated doses expressed in molarity (M). DX3 cell viability was evaluated by XTT assay after 24 (panel A ), 48 (panel B ) and 72 hours (panel C ). Panels D – F show WM-115 cell viability evaluated as described above after 24, 48 and 72 hours respectively. Graphic bars represent percentage of living cells in each sample. The results have been normalized to 0.1% DMSO treated cells and are the means of three independent experiments ± s.d. * P -value < 0.001 versus 0.1% DMSO treated cells.

Journal: Oncotarget

Article Title: Antiproliferative and pro-apoptotic activity of melatonin analogues on melanoma and breast cancer cells

doi: 10.18632/oncotarget.20124

Figure Lengend Snippet: DX3 and WM-115 cells were seeded as described in Materials and Methods and treated with 0.1% DMSO, melatonin (MLT), UCM 976, UCM 1032, UCM 1033 and UCM 1037 dissolved in 0.1% DMSO at the indicated doses expressed in molarity (M). DX3 cell viability was evaluated by XTT assay after 24 (panel A ), 48 (panel B ) and 72 hours (panel C ). Panels D – F show WM-115 cell viability evaluated as described above after 24, 48 and 72 hours respectively. Graphic bars represent percentage of living cells in each sample. The results have been normalized to 0.1% DMSO treated cells and are the means of three independent experiments ± s.d. * P -value < 0.001 versus 0.1% DMSO treated cells.

Article Snippet: The cell viability and proliferation were assayed using the XTT Cell Viability Assay Kit (Cell Signaling, Euroclone, Milan, Italy), according to the manufacturer's protocol.

Techniques: XTT Assay

MCF-7 and MDA-MB231 cells were seeded as described in Materials and Methods and treated with 0.1% DMSO, melatonin (MLT), UCM 976, UCM 1032, UCM 1033 and UCM 1037 dissolved in 0.1% DMSO at the indicated doses expressed in molarity (M). After 24 (panel A ), 48 (panel B ) and 72 hours (panel C ) MCF-7 cell viability was evaluated by XTT assay. Panels D – F show MDA-MB231 cell viability evaluated as described above after 24, 48 and 72 hours respectively. Graphic bars represent percentage of living cells in each sample. The results have been normalized to 0.1% DMSO treated cells and are the means of three independent experiments ± s.d. * P -value < 0.001 versus 0.1% DMSO treated cells.

Journal: Oncotarget

Article Title: Antiproliferative and pro-apoptotic activity of melatonin analogues on melanoma and breast cancer cells

doi: 10.18632/oncotarget.20124

Figure Lengend Snippet: MCF-7 and MDA-MB231 cells were seeded as described in Materials and Methods and treated with 0.1% DMSO, melatonin (MLT), UCM 976, UCM 1032, UCM 1033 and UCM 1037 dissolved in 0.1% DMSO at the indicated doses expressed in molarity (M). After 24 (panel A ), 48 (panel B ) and 72 hours (panel C ) MCF-7 cell viability was evaluated by XTT assay. Panels D – F show MDA-MB231 cell viability evaluated as described above after 24, 48 and 72 hours respectively. Graphic bars represent percentage of living cells in each sample. The results have been normalized to 0.1% DMSO treated cells and are the means of three independent experiments ± s.d. * P -value < 0.001 versus 0.1% DMSO treated cells.

Article Snippet: The cell viability and proliferation were assayed using the XTT Cell Viability Assay Kit (Cell Signaling, Euroclone, Milan, Italy), according to the manufacturer's protocol.

Techniques: XTT Assay

NIH3T3, NIH3T3-1A and NIH3T3-1B cells were treated with 0.1% DMSO or with UCM 1037 10 −4 M dissolved in 0.1% DMSO. After 24, 48 and 72 hours cell viability was evaluated by XTT assay ( A ) and Trypan blue dye exclusion assay ( B ). In panel A bar graph represents the percentages of living cells normalized to 0.1% DMSO treated cells. In panel B bar graph represents the percentage of dead cells in each sample. Data are the means of three independent experiments ± s.d. * P -value < 0.001 versus 0.1% DMSO treated cells.

Journal: Oncotarget

Article Title: Antiproliferative and pro-apoptotic activity of melatonin analogues on melanoma and breast cancer cells

doi: 10.18632/oncotarget.20124

Figure Lengend Snippet: NIH3T3, NIH3T3-1A and NIH3T3-1B cells were treated with 0.1% DMSO or with UCM 1037 10 −4 M dissolved in 0.1% DMSO. After 24, 48 and 72 hours cell viability was evaluated by XTT assay ( A ) and Trypan blue dye exclusion assay ( B ). In panel A bar graph represents the percentages of living cells normalized to 0.1% DMSO treated cells. In panel B bar graph represents the percentage of dead cells in each sample. Data are the means of three independent experiments ± s.d. * P -value < 0.001 versus 0.1% DMSO treated cells.

Article Snippet: The cell viability and proliferation were assayed using the XTT Cell Viability Assay Kit (Cell Signaling, Euroclone, Milan, Italy), according to the manufacturer's protocol.

Techniques: XTT Assay, Exclusion Assay